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Protein
Purification:
Principles and Practice
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TECHNIQUES
AND INSTUMENTS YOU WILL USE
- Tissue Homogenizers
(Omni International)
- Filtration Devices including Tangential
Flow Ultrafiltration (Millipore, Sartorius, Vivascience,
- and introducing the Minimate TFF
System, by Pall Life Sciences)
- Refrigerated (Sorvall)
Centrifuges
- Recording UV-Vis Spectrophotometers
(Spectronic, Cary)
- Filter Fluorometers (Turner Designs, GE Healthcare/Hoefer)
- Chromatography: Gel Filtration,
Ion-Exchange, & Hydrophobic Interaction (GE Healthcare, BioRad)
- Immobilized Metal Ion Affinity Chromatography
(Qiagen, BD Biosciences/Clontech)
- Fraction Collectors (Gilson, ISCO)
- Column Monitors
- Electrophoresis (SDS, native and
isoelectric focusing, 2D) (BioRad, Invitrogen/Novex, GE Healthcare)
- Western Blotting (Invitrogen/Novex)
- Coomassie Blue and Silver Staining
(Sigma-Aldrich)
- Ion Exchange HPLC (Applied Biosystems)
- Size Exclusion HPLC (Thermo
Electron Corporation, Phenomenex)
- GE Healthcare Phast Electrophoresis
System
- Molecular Modeling
- Amino Acid Analysis*
- Automated Edman Sequencing*
- DNA Sequence Analysis*
- Peptide Mapping*
- Mass Spectral Analysis*
*Introduced
in problem-solving workshop, Friday
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