Lectures for Advanced
Virology
Happy Halloween - ratmonster
Lecture for General Micro
Schedule
Comparative
Virology Fall, 2005
This
schedule will change in the course of the semester, and will be updated.
1. Fri Sept. 2 Course
introduction; history and general properties of viruses (BH)
Chapter 1
Preston
– Selected audio clips from “The Demon in the Freezer”
2. Tue Sept. 6 History
& properties, cont’d
Chapter
3 – read text, scan figures
Chapter
4 – read text, scan figures. Pay attention to
3. Fri Sept.
9 Virus
structure
4. Tue Sept. 13 Virus
nomenclature (BH)
5. Fri Sept. 16 Techniques
used in virus research (BH)
pp. 27- first column of
46
6. Tue Sept. 20 DNA Viruses 1: Adenovirus
(HAV1) Poxvirus (Smallpox) (BH)
7. Fri Sept. 23 DNA Viruses 2: Papovavirus (SV40), Herpesvirus (HSV1) (BH), Presentation : Sara, Sal
8.
Tue Sept. 27 Quiz
1; Begin RNA properties and RNA viruses,
transcription (NT)
Chapter
8
9. Fri Sept 30 General properties of virus translation
(NT) Presentation:
Roger,
Devin
Chapter 11
10.Tue Oct.
4 General properties of virus replication
(NT) Presentation:
Craig,
Scott
Chapter 6
11. Fri Oct. 7 Positive sense RNA
viruses of animals – Poliovirus,
West Nile virus (NT) Presentation: Wendy,
Cheryl
12. Tue
Oct. 11 Positive
sense RNA viruses of plants - BMV,
TMV (NT)
13. Fri Oct. 14 Quiz 2; Retroviruses 1 – (NT) Presentation: Ummais, Ruchi
14. Tue Oct. 18 Retroviruses 2 – (NT) Presentation: Vijaya,
Neelam
Chapter 7
15. Fri Oct. 21 Viral
transformation; oncogenesis; Hepatitis C virus
(NT)
Presentation: Varsha, Jeni
16. Tue
Oct. 25 Pararetroviruses (CaMV), Hepadnavirus (HBV) and retrotransposons (BH)
17.
Fri Oct. 28 Negative
sense RNA viruses – Rabies, measles, (BH) Presentation: Mohan,
Karan
18. Tue
Nov. 1 Quiz
3; Negative
sense RNA viruses – Hantavirus, influenza (BH) Presentation:
Christie,
Hemal
19.
Fri Nov. 4 dsRNA
viruses (BH) Presentation: Remi, Jazmin
Good
review article for negative sense RNA viruses
20. Tue Nov. 8 Host
defense against virus infection (NT) Presentation: Kate,
Andrew
21. Fri Nov. 11 Antivirals, vaccines, and vaccine technology (NT) Presentation: Ambrish, Yuri
22. Tue Nov. 15 Viroids,
Satellites and Defective Viruses; Prions,
mad
cow disease (BH)
23. Fri Nov. 18 Quiz
4; Use
of viruses for biological control; Baculovirus; Polydnavirus; Fungal Viruses; Bacteriophages
(BH)
24. Tue Nov. 29 Use
of viruses for gene therapy (NT); Manufacturing
antiviral vaccines in plants (Nikolai Borisjuk,
25. Fri Dec. 2 Genetic
engineering to protect against virus infection (NT) Presentation: Mei,
Parth
26. Tue
Dec. 6 Virus
ecology and evolution (BH) Presentation: Vrushali, Jon
27. Fri Dec. 9 Virus ecology and evolution (BH) Presentation: Santy,
James
28. Tue Dec
13 Quiz 5
Final
exam: (Scheduled for Friday December 23,
Note: This schedule will be modified as the semester progresses. You will be given sufficient warning of any major changes.
Grades
A total of 500 points are possible:
Quizzes 100 points each - count best 3 out of the first 4, plus # 5
Presentation 100 points
Class
Presentations
Each student in the course will participate in a group
presentation (2-3 students per group), responsible for covering a focused topic
area in a 10-15 minute presentation. Each group will provide a 4-5 page,
double-spaced paper summarizing the presentation, and will provide copies of
material used for the presentation (e.g., overheads or PowerPoint slides). At
least 5 references will be required, in the approved format as described below.
A minimum of two of the references must be from refereed journal articles. All of the relevant information in your paper
should be referenced. In other words, if you make a statement that is not based
on information that is common knowledge or that was covered in class, the
reference for the statement should be cited in your paper, and we should be
able to find the information*. Your presentation outline must be cleared with
the instructors at least two weeks before the presentation. This means that you
should do a literature search, download the articles you intend to use, and
discuss them with one of the instructors at least two weeks before the
presentation. Specifics of the presentation must be cleared at least one week
before the presentation, and a file containing the presentation must be
provided by
Format for references in papers:
Hillman, B. I., Foglia, R., and
Yuan, W. 2000. Satellite and defective RNAs of Cryphonectria
hypovirus 3, a virus species in the Family Hypoviridae
with a single open reading frame. Virology 276, 181-189.
Hudak, K., Wang, P. and Tumer, N. E. 2000. Pokeweed
antiviral protein inhibits translation of capped mRNAs independently of
ribosome depurination by acting directly on the RNA template. RNA 6,
1-12.
Citations to papers in text should be of the form: (Hillman et
al., 2000; Hudak et al., 2000), for example.
*A note about plagiarism: It turns out that your
instructors are both pretty honest individuals who rose to their current
positions as
Possible topics for presentations:
Emerging flaviviruses, Japanese encephalitis virus
and Dengue virus
Hepatitis C virus epidemiology
Origin and evolution of hepatitis viruses
Foot and Mouth disease epidemic 1999-2001
SARS
The cruise ship virus,
Yellow fever
LaCrosse virus
Hantavirus Disease
Potential for influenza pandemic - Avian flu H5N1
Production of influenza vaccines
Viral hemorrhagic fevers, Junin virus, Machupo virus, Lassa
Fever
Filoviruses, Ebola and
Origin of HIV
Should Smallpox virus stocks be destroyed?
Emerging plant viruses, Tomato yellow leaf curl virus
Citrus tristeza virus
Transgenic plants for control of Papaya ringspot virus
Plant viruses and gene silencing
Viruses of thermophylic organisms
Think about possible presentation topics you’d like to do
over the Labor Day weekend. We’ll begin scheduling these presentations Tuesday
Sept. 6 and will complete scheduling Friday Sept. 9. The first presentation
will be Friday Sept. 16.
Some useful web sites:
General
http://www.virology.net/garryfavweb2.html#viraltax
Virus Taxonomy and Nomenclature
http://www.virustaxonomyonline.com/
http://www.danforthcenter.org/iltab/ICTVnet/asp/_MainPage.asp
http://www.ncbi.nlm.nih.gov/ICTVdb/
Virus Structure
VIPER http://mmtsb.scripps.edu/viper/
Viruses: Structure to Biology http://medicine.wustl.edu/~virology/
Virus Sequence Information
Viruses that you should be familiar with by the end of the
course:
Poliovirus
(Picornaviridae)
Hepatitis C virus
(Flaviviridae)
Tobacco
mosaic virus (Tobamovirus)
Brome mosaic virus
(Bromoviridae)
Tobacco etch virus
(Potyviridae)
Rabies virus
(Rhabdoviridae)
Measles virus
(Paramyxoviridae)
Ebola virus (Filoviridae)
Hantavirus (Bunyaviridae)
Influenza virus
(Orthomyxoviridae)
Bluetongue virus
(Reoviridae)
Human immunodeficiency
virus 1 (Retroviridae)
Cauliflower mosaic virus
(Pararetrovirus – Caulimoviridae)
Hepatitis B virus
(Hepadnaviridae)
Tomato yellow leaf curl
virus (Geminiviridae)
Human adenovirus 1
(Adenoviridae)
Simian virus 40
(Polyomaviridae)
Human herpesvirus 3 (= Varicella-zoster virus, chicken pox; Herpesviridae)
Smallpox virus
(Poxviridae)
Baculovirus
(Baculoviridae)
Polydnavirus
(Polydnaviridae)
Potato spindle tuber
viroid (Pospiviroid)
Bovine spongiform
encephalopathy prion
Some questions you should be able to answer by the end of
the course:
What are the major types of viruses described in this
course? What are the main features of their replication, biology, and
pathology?
What are some of the major weapons that viruses use to
compromise and facilitate infection in their hosts?
What are some of the different mechanisms that various host
organisms use to protect themselves against virus infection?
What are the major means by which viruses are transmitted
from an infected host to a healthy host?
How do viruses move within different host organisms?
What are some of the major properties of virus structure and
composition?
What are some of the major features of virus evolution?