than 2000 scientists from around the world have strongly recommended
this intensive course as an opportunity to develop protein research
and analytical skills in a retreat setting. Participants work
hard, identify and solve problems in the lab and enjoy comaraderie
and good food and drink with colleagues.
and one half day laboratory course covers a wide variety of conventional
methods for protein isolation, purification, and characterization.
The course format integrates hands-on laboratory exercises with
classroom lectures, demonstrations, study breaks, and short take-home
feature of the course is that all laboratory work will be performed
on the same starting sample (Aequorea GFP or recombinant
GFP), which will be purified from an exceedingly crude form (starting
with tissue or bacterial cell extraction) to near homogeneity
as judged by high performance liquid chromatography (HPLC), SDS
gel electrophoresis, isoelectric focusing, and western blotting.
This feature provides a continuity of purpose, integrating dozens
of preparative and analytical protein techniques in a way that
few competing courses can match.
approach will be used throughout the course. Under the guidance
of experienced lab instructors, participants will work in groups
of three to plan their own protocols, analyze data, and interpret
results. A student-teacher ratio not greater than 8:1 will be
maintained and the faculty coordinators will be present throughout