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The Center for Research and Education
in Bioluminescence and Biotechnology


Protein Purification:
Principles and Practice

Protein Lab


A 5 ½ day hands-on laboratory course in which participants will learn skills to effectively
design separation and purification strategies for downstream processing. This course
uses the
remarkable Green-Fluorescent Protein (GFP),
a novel marker for gene expression,
as the source material.
While this is a general course applicable to all proteins,
we use GFP as a highly effective visual teaching tool.

2014 Dates

5 1/2 - Day Principles in

Protein Purification Course

May 4 - 9, 2014


Hurry up, as summer registration fills in quickly!!



More than 2000 scientists from around the world have strongly recommended this intensive course as an opportunity to develop protein research and analytical skills in a retreat setting. Participants work hard, identify and solve problems in the lab and enjoy comaraderie and good food and drink with colleagues.

This five and one half day laboratory course covers a wide variety of conventional methods for protein isolation, purification, and characterization. The course format integrates hands-on laboratory exercises with classroom lectures, demonstrations, study breaks, and short take-home assignments.

A special feature of the course is that all laboratory work will be performed on the same starting sample (Aequorea GFP or recombinant GFP), which will be purified from an exceedingly crude form (starting with tissue or bacterial cell extraction) to near homogeneity as judged by high performance liquid chromatography (HPLC), SDS gel electrophoresis, isoelectric focusing, and western blotting. This feature provides a continuity of purpose, integrating dozens of preparative and analytical protein techniques in a way that few competing courses can match.

A problem-solving approach will be used throughout the course. Under the guidance of experienced lab instructors, participants will work in groups of three to plan their own protocols, analyze data, and interpret results. A student-teacher ratio not greater than 8:1 will be maintained and the faculty coordinators will be present throughout the course.

Course Location, Hotel & Transportation Information  Hotel & Transportation PDF(click here)

The course will be held at Lipman Hall on the Cook College Campus, New Brunswick, NJ and a PDF map of the area can be found at or In addition, a map with written directions to the course site will be included with your confirmation of registration. If you do not receive your confirmation ten days before the course starts, please call our Registration Desk at (732) 932-9562 ext.225.

Our full address is:

CREBB Registration Desk
School of Environmental and Biological Sciences,
Rutgers University
Department of Biochemistry and Microbiology
76 Lipman Drive
New Brunswick, NJ 08901-8525

Lipman Hall
         We're located approx. 40 min. South of NYC Pictures of our Cook College Location

Information about New Jersey and New Brunswick,NJ

Lodging and Travel Information

For information on discounted lodging and travel, please visit the following Rutgers link:

2008 Local Hotel Rates:

Hotel Discounts for Academic Registrants
Students/teachers hotel rebate site:
New Brunswick hotels rebate page

For convenient on-campus accommodations within easy walking distance,
we recommend the University Inn and Conference Center

The Center for Research and Education in Bioluminescence and Biotechnology (CREBB)

The Center offers a series of continuing education workshops each year featuring nationally renowned presenters. The Center for Research and Education in Bioluminescence and Biotechnology (CREBB) is a component of Rutgers University, School of Environmental and Biological Sciences. The CREBB mission is to perform basic research on bioluminescence and to utilize bioluminescence (especially the Green-Fluorescent Protein) as a tool to educate the scientific and industrial communities in the field of biotechnology.

Rutgers Logo
Faculty Coordinator

Dr. William W. Ward
Associate Professor of Biochemistry
School of Environmental and Biological Sciences, Rutgers University
and Director of C.R.E.B.B.