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The Center for Research and Education
in Bioluminescence and Biotechnology
Presents:
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BIOCHEMICAL
SEPARATIONS:
An Introduction to Laboratory Techniques in Biotechnology
A Two Day Lecture/Demonstration
Course
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2009 Dates
Please check back for new dates.
Courses are Friday to Saturday
Friday 9am - 6pm
Saturday 8am - 5pm
Please
inquire about arranging
a personalized hands-on tutorial
- groups are especially welcome
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We also offer this
course for companies/groups, on-site or off-site at your convenience.
Course Description
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This is an introductory-level
course designed to acquaint participants with the wide range of
modern techniques available for separating and purifying biomolecules.
The fundamentals of each technique will be presented, including
practical examples; however, sufficient theoretical background
will be provided to enable the participants to understand how
each technique functions. Emphasis will be placed on techniques
used in protein isolation and purification-both from native and
recombinant sources. The course will be particularly valuable
to those who are beginning work in biochemistry and biotechnology
laboratories and who wish to broaden their background in separation
procedures used in biochemistry. "Biochemical Separations" may
be useful for persons getting back into the laboratory after several
years, for those between jobs, and for scientists whose job description
now requires familiarity with separation techniques used in Downstream
Processing. |
INTRODUCTION TO THE PROPERTIES
OF BIOMOLECULES
Chemical and
physical properties of proteins, nucleic acids, carbohydrates, lipids,
and other biomolecules.
General strategies for molecular separations will be introduced.
PREPARATIVE
METHODS
Survey of batch methods (salting out, isoelectric
recipitation, and batch adsorption), dialysis, filtration, ultrafiltration.
Selected methods used in enzyme purification will be presented in
detail.
BASICS
OF LIQUID CHROMATOGRAPHY OF BIOMOLECULES
Principles of liquid chromatography. Emphasis will
be placed on modern methods of low pressure liquid chromatography
including gel filtration chromatography. Elementary chromatographic
theory will be introduced.
ION EXCHANGE AND HYDROPHOBIC
INTERACTION CHROTOGRAPHY
A survey of ion exchange and hydrophobic (reverse phase) separation
methods for proteins, nucleic acids and smaller molecules. Examples
will include both high pressure and low pressure methods.
AFFINITY
CHROMATOGRAPHY
An introduction to this powerful method for purifying enzymes, antibodies,
nucleic acids, and polysaccharides. Examples will include immobilized
metal ion affinity chromatography, and affinity chromatography of
enzymes with immobilized substrate analogues. Separation of recombinant
proteins will be emphasized.
CONTEMPORARY
PRACTICE OF LIQUID CHROMATOGRAPHY (HPLC)
An overview of the basic operating principles of HPLC with a comparison
to other available tools. Special emphasis will be placed on the role
of modem liquid chromatography as a problem-solving tool for bioanalytical
research.
FUNDAMENTALS
OF ELECTROPHORESIS
Basic principles of electrophoresis and survey of electrophoretic
methods. Emphasis will be placed on polyacrylamide gel electrophoresis
of proteins, molecular weight estimation by SDS PAGE, and isoelectric
focusing
SEPARATION
TECHNIQUES IN PROTEIN AND DNA SEQUENCING
Use of HPLC reverse phase chromatography in the sequencing
of proteins by automated Edman degradation. Discussion of electrophoresis
of nucleic acids as it applies to the sequencing of DNA.
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Visit
the website for our 5 1/2 day laboratory course on
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One can register by credit card (VISA or Mastercard) or by check (business
or personal) or money order. The tuition fee includes continental
breakfasts, lunches, and coffee breaks along with all course materials
and hand-outs. Tuition Fees MUST BE PAID at least 3 weeks prior to
the start of the course to be eligible for the discounted early registration
rates.
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By
Phone
9:00
a.m. - 4:00p.m., Monday-Friday
Registration Desk: (732) 932-9763 ext. 212
By Mail
Send check or money order (payable to Rutgers, the State University)
plus completed registration PDF
form
to:
Registration
Desk- Biochemical Separations
CREBB, Rutgers
University,
School
of Environmental and Biological Sciences
Department of Biochemistry and Microbiology
76 Lipman Drive
New Brunswick, NJ 08901-8525
By Fax
(732) 932-3633, 24 hours
Please
fax a copy of check or money order with fax registration.
Refunds
You may withdraw from this course with a full refund (less a
$100
processing
fee) provided our office is notified at least five (10) full
business days prior to the start of the course. Beyond that
time, you may be responsible for the full tuition fee if you
register but do not attend. Substitutions are encouraged.
CREBB
reserves the right to cancel this course due to insufficient
enrollment. |
| Course
Location, Hotel & Transportation Information
(click
here)
The
course will be held at Lipman Hall on the Cook College Campus,
New Brunswick, NJ and a PDF map of the area can be found
at http://maps.rutgers.edu/maps/default.aspx?campus=6
or http://maps.rutgers.edu/pdf/CookDouglass.pdf.
In addition, a map with written directions to the course
site will be included with your confirmation of registration.
If you do not receive your confirmation ten days before
the course starts, please call our Registration Desk at
(732) 932-9562 ext.225. |
Our
full address is:
CREBB
Registration Desk
School of Environmental and Biological Sciences,
Rutgers University
Department of Biochemistry and Microbiology
76 Lipman Drive
New Brunswick, NJ 08901-8525 |
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Information
about New Jersey and New Brunswick,NJ
http://www.aboutnewjersey.com/
http://www.aboutnewjersey.com/Regions/Capital/Middlesex/NewBrunswick/index.php
http://newbrunswick.com/
Lodging and Travel Information
For
information on discounted lodging and travel, please visit the
following Rutgers link: http://www.rutravel.rutgers.edu/discountprograms.html
2008 Local Hotel Rates: http://www.rutravel.rutgers.edu/hotelrates2008.html
Hotel
Discounts for Academic Registrants
Students/teachers hotel rebate site: http://www.hotelscombined.com/Education_Support
New Brunswick hotels rebate page
http://www.hotelscombined.com/City/New_Brunswick.htm
For convenient on-campus accommodations within easy walking distance,
we recommend the University
Inn and Conference Center.
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Faculty Coordinator/Instructor
Dr.
William W. Ward
Associate
Professor of Biochemistry
School
of Environmental and Biological Sciences,
Rutgers University
and Director of C.R.E.B.B.
Dr. Ward is
a noted teacher and seminar leader with more than 20 years
of experience with adult professional audiences. He specializes
in the chemical and physical properties of the green-fluorescent
protein and other proteins involved in marine bioluminescence.
Dr. Ward also teaches "Fluorescence: Basic Principles and
Applications in Drug Discovery" for IBC Life Sciences Training
Academy.
Dr. Ward has
co-authored (with Catherine Thomson, Ph.D.) "A Guide to Green-Fluorescent
Protein: Applications in Cell Biology and Drug Discovery"
for D&MD Publications. This resource-oriented practical guide
presents an overview of the most important features and technological
applications of Green-Fluorescent Protein (GFP) and its variant
forms, and illustrates how GFP is currently being used in
the biopharmaceutical industry.
D&MD Publishing
is happy to extend a 10% discount to the registrants of the
Protein Purification course and Biochemical Separations course.
When ordering this Guide, please mention source code "9133-10%PP".
To order the Guide, please contact D&MD's Customer Service
Department at cust.serv@drugandmarket.com or call (508)616-5566.
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Professor
William W. Ward (Director CREBB) at (732) 932-9763 ext. 216 or e-mail
at crebb@rci.rutgers.edu
or
Randy Ward (Program Coordinator) at (732) 932-9763 ext. 212 or e-mail
at rward@rci.rutgers.edu
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