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The Center for Research and Education
in Bioluminescence and Biotechnology
Presents:
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BIOCHEMICAL
SEPARATIONS:
An Introduction to Laboratory Techniques in Biotechnology
A Three Day Lecture/Demonstration
Course
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2008
Course Dates
May 16-18, 2008
September 19-21, 2008
All courses are Friday thru Sunday
Friday 3pm - 7pm
Saturday 9am - 5pm
Sunday 10am - 3pm
Please
inquire about arranging
a personalized 2 day hands-on tutorial
- groups are especially welcome
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We also offer this
course for companies/groups, on-site or off-site at your convenience.
Course Description
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This is an introductory-level
course designed to acquaint participants with the wide range of
modern techniques available for separating and purifying biomolecules.
The fundamentals of each technique will be presented, including
practical examples; however, sufficient theoretical background
will be provided to enable the participants to understand how
each technique functions. Emphasis will be placed on techniques
used in protein isolation and purification-both from native and
recombinant sources. The course will be particularly valuable
to those who are beginning work in biochemistry and biotechnology
laboratories and who wish to broaden their background in separation
procedures used in biochemistry. "Biochemical Separations" may
be useful for persons getting back into the laboratory after several
years, for those between jobs, and for scientists whose job description
now requires familiarity with separation techniques used in Downstream
Processing. |
INTRODUCTION TO THE PROPERTIES
OF BIOMOLECULES
Chemical and
physical properties of proteins, nucleic acids, carbohydrates, lipids,
and other biomolecules.
General strategies for molecular separations will be introduced.
PREPARATIVE
METHODS
Survey of batch methods (salting out, isoelectric
recipitation, and batch adsorption), dialysis, filtration, ultrafiltration.
Selected methods used in enzyme purification will be presented in
detail.
BASICS
OF LIQUID CHROMATOGRAPHY OF BIOMOLECULES
Principles of liquid chromatography. Emphasis will
be placed on modern methods of low pressure liquid chromatography
including gel filtration chromatography. Elementary chromatographic
theory will be introduced.
ION EXCHANGE AND HYDROPHOBIC
INTERACTION CHROTOGRAPHY
A survey of ion exchange and hydrophobic (reverse phase) separation
methods for proteins, nucleic acids and smaller molecules. Examples
will include both high pressure and low pressure methods.
AFFINITY
CHROMATOGRAPHY
An introduction to this powerful method for purifying enzymes, antibodies,
nucleic acids, and polysaccharides. Examples will include immobilized
metal ion affinity chromatography, and affinity chromatography of
enzymes with immobilized substrate analogues. Separation of recombinant
proteins will be emphasized.
CONTEMPORARY
PRACTICE OF LIQUID CHROMATOGRAPHY (HPLC)
An overview of the basic operating principles of HPLC with a comparison
to other available tools. Special emphasis will be placed on the role
of modem liquid chromatography as a problem-solving tool for bioanalytical
research.
FUNDAMENTALS
OF ELECTROPHORESIS
Basic principles of electrophoresis and survey of electrophoretic
methods. Emphasis will be placed on polyacrylamide gel electrophoresis
of proteins, molecular weight estimation by SDS PAGE, and isoelectric
focusing
SEPARATION
TECHNIQUES IN PROTEIN AND DNA SEQUENCING
Use of HPLC reverse phase chromatography in the sequencing
of proteins by automated Edman degradation. Discussion of electrophoresis
of nucleic acids as it applies to the sequencing of DNA.
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Visit
the website for our 5 1/2 day laboratory course on
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| Tuition
Fee: |
$895 (Early registration paid 3 weeks prior
to start of course)
$850 (Multiple
registration 2 or more attendees from the same company)
$995 (Late
registration)
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One can register by credit card (VISA or Mastercard) or by check (business
or personal) or money order. Register three weeks prior to the start
of the course with payment and pay only $895. A discounted fee of
$850 per person is available if two or more registrants from the same
company register. The tuition fee includes continental breakfasts,
lunches, and coffee breaks along with all course materials and hand-outs.
Tuition Fees MUST BE PAID at least 3 weeks prior to the start of the
course to be eligible for the discounted early registration rates.
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By
Phone
9:00
a.m. - 4:00p.m., Monday-Friday
Registration Desk: (732) 932-9763 ext. 225
By Mail
Send check or money order (payable to Rutgers, the State University)
plus completed registration PDF
form
to:
Registration
Desk- Biochemical Separations
CREBB, Rutgers
University,
School
of Environmental and Biological Sciences
Department of Biochemistry and Microbiology
76 Lipman Drive
New Brunswick, NJ 08901-8525
By Fax
(732) 932-3633, 24 hours
Please
fax a copy of check or money order with fax registration.
Refunds
You may withdraw from this course with a full refund (less a
$100
processing
fee) provided our office is notified at least five (5) full
business days prior to the start of the course. Beyond that
time, you may be responsible for the full tuition fee if you
register but do not attend. Substitutions are encouraged.
CREBB
reserves the right to cancel this course due to insufficient
enrollment. |
| Course
Location, Hotel & Transportation Information
(click
here)
The course will be held at Lipman Hall on the Cook
College Campus, New Brunswick, NJ and a PDF map of the area can
be found at http://maps.rutgers.edu/maps/default.aspx?campus=6
or http://maps.rutgers.edu/building.aspx?190.
In addition, a map with written directions to the course site
will be included with your confirmation of registration. If you
do not receive your confirmation ten days before the course starts,
please call our Registration Desk at (732) 932-9562 ext.225.
Our
full address is:
CREBB
Registration Desk
School
of Environmental and Biological Sciences,
Rutgers University
Department of Biochemistry and Microbiology
76 Lipman Drive
New Brunswick, NJ 08901-8525 |
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Lodging and Travel Information
For
information on discounted lodging and travel, please visit the
following Rutgers link: http://www.rutravel.rutgers.edu/discountprograms.html
For convenient on-campus accomodations within easy walking distance,
we recommend the University
Inn and Conference Center.
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For any more information about the course please contact:
Professor William W. Ward (Director) at (732) 932-9562 ext. 212 or e-mail
at crebb@rci.rutgers.edu
or
Gloria Kierniesky (Program Coordinator) at (732) 932-9562 ext. 225,
or e-mail at kierniesky@aesop.rutgers.edu
Faculty Coordinator/Instructor
Dr.
William W. Ward
Associate
Professor of Biochemistry
School
of Environmental and Biological Sciences,
Rutgers University
and Director of C.R.E.B.B.
Dr. Ward is
a noted teacher and seminar leader with more than 20 years
of experience with adult professional audiences. He specializes
in the chemical and physical properties of the green-fluorescent
protein and other proteins involved in marine bioluminescence.
Dr. Ward also teaches "Fluorescence: Basic Principles and
Applications in Drug Discovery" for IBC Life Sciences Training
Academy.
Dr. Ward has
co-authored (with Catherine Thomson, Ph.D.) "A Guide to Green-Fluorescent
Protein: Applications in Cell Biology and Drug Discovery"
for D&MD Publications. This resource-oriented practical guide
presents an overview of the most important features and technological
applications of Green-Fluorescent Protein (GFP) and its variant
forms, and illustrates how GFP is currently being used in
the biopharmaceutical industry.
D&MD Publishing
is happy to extend a 10% discount to the registrants of the
Protein Purification course and Biochemical Separations course.
When ordering this Guide, please mention source code "9133-10%PP".
To order the Guide, please contact D&MD's Customer Service
Department at cust.serv@drugandmarket.com or call (508)616-5566.
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Professor
William W. Ward (Director CREBB) at (732) 932-9562 ext. 212 or e-mail
at crebb@rci.rutgers.edu
or
Gloria Kierniesky (Program Coordinator) at (732) 932-9562 ext. 225,
or e-mail at kierniesky@aesop.rutgers.edu
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