155: 411 INTRODUCTION TO BIOCHEMICAL ENGINEERING Fall 1998
Department of Chemical and Biochemical Engineering, Rutgers University
Instructor: Professor Prabhas V. Moghe
Course Outline
• Introduction to Biochemical Engineering and Biotechnology
Unique features of the field; Definitions and Concepts; Classification of Microbial Species; Scope of Biotechnology, and Survey of Existing Industrial Products
• Stoichiometry and Energetics of Microbial Growth
Introduction of organisms as mass and energy consumers; Elemental balance equations of growth; Metabolic coupling of ATP and NAD+; Yield Coefficients.
• Unstructured growth models
Phases of batch growth cycle; Monod Model; Models of Product Formation and Inhibition; Introduction to structured models.
• Bioreactor Design
Batch reactors; Continuous Stirred Tank Bioreactors (Chemostat); Enzyme Catalysis in Chemostat; Age Distribution Models; Fed Batch Reactors; Introduction to Transient Bioreactor Behavior and the Stability Criteria; Recycle Systems.
• Transport Phenomena
Rheology; Gas-Liquid Mass Transfer- Measurement of transfer coefficients; Design of bubble columns; Treatment of Agitated Tanks- Mixing and Power Requirements; Scale-up Issues.
• Sterilization
Kinetics of thermal death of microorganisms; Batch and Continuous Sterilizers.
• Bioseparations
Downstream Processing- Scope and Challenges; Removal of product - Filtration, Settling and Centrifugation; Homogenization; Isolation of product - Extraction; Purification - Precipitation, Adsorption, Chromatography; Polishing - Crystallization and Drying