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Dr. Gleba's 25 years of research experience in plant
physiology, genetics and plant cell culture is reflected in more
than 50 peer-review journal publications and presentations. She
graduated from Vilnius State University, Lithuania, in 1971, with a
M.Sc. in biology-chemistry and in 1979 received her Ph.D. in plant
genetics from the Institute of Plant Physiology, USSR Academy of
Sciences in Moscow. Her Ph.D. project was focused on the development
of technology of suspension culture of Dioscorea deltoidea Wall.
cells source of secondary metabolites- saponins.
After graduation, Dr. Gleba established a Plant Tissue
Laboratory at the Institute of Botany, Academy of Sciences, Lithuania and
worked as a Research Scientist developing methods of plant cell
culture technologies such as rapid regeneration, mutant isolation
and media optimization for plant breeding.
In 1979 she worked as a Research Scientist in the Laboratory of
Plant Tissue Cultures at All-Union Institute of Sugar-Beet Breeding
in Kiev, Ukraine , developing new varieties of sugar beets using new
technologies - culture of isolated protoplasts, microspore culture
as somatic hybridization.
During 1981-1984, as Senior Research Scientist at Botanical
Garden of the Kiev State University, she was responsible for
microclonal propagation and biotechnology’s of medicinal
plants, and was a member of Scientific Council of Biological faculty
and Botanical garden. Dr Gleba also participated in student teaching
process as a supervisor for student diploma projects.
In 1990-1991 she was a post-doctoral trainee at Institute of
Pharmaceutical Biology , Munich University in Germany were her
research involved tissue cultures of species- producers of novel
pharmaceuticals (Croton, Taxus).
As a Post-Doctoral Associate in Dr Raskin Laboratory at Rutgers
University, she was involved in various Phytoremediation projects.
In particular, she developed methods of plant screening for higher
metal uptake as well as genetic improvement of plants for
phytoremediation using somatic plant hybridization. Dr. Gleba was
involved in all aspects of research, starting with experimental
design, carrying out experiments, data analysis and
recommending future research ; she presented results at regular
laboratory meetings, Biotech seminars and at Scientific Advisors
Meetings at Phytotech, Inc.
Based on her extensive knowledge in plant physiology,
microbiology and plant cell culture, Doloressa successfully
developed methods of screening plant leave cuticle washings, plant
root extracts and exudates for antimicrobial and antifungal activity
for new drug discovery .
Dr. Gleba's main research interests are in the scientific field
of plant cell biology and genetics; plant physiology;
phytoremediation; plant microclonal propagation and plant
biotechnologies for conservation of biodiversity; natural
products.
Recent Publications
Gleba D., Momot V., Okolot A., Gleba Y.,
1985. Effect of para-flurophenyl alanine on nuclear DNA content and
chromosome segregation in hybrid cells of Nicotiana+ Atropa.
Cytology and Genetics, 19, p. 281-285.
Rudzianskaite A., Gleba D., Cieminis K. 1985.
Incorporation 3H-thymidine in tobacco protoplasts. Proc. Acad. Sci.
Lithuanian SSR, 17, p.1102-1104.
Gleba D. 1987. Use in vitro tissue culture methods in
plant introduction. Abstr. 8 Ukrainian Botanical Society, Kiev,
p.209.
Gleba D., Gleba Y. 1987. Hybridization
of somatic plant cells. Abstr. 5 All-Union Soc. of geneticists and
breeders. Vol. VI, p. 131.
Gleba D.,Gordzievskaya L. 1987. Propagation of Platycerium
bifurcatum(Cav) in in vitro culture.
Abstr.Conf. "Introduction and acclimatization of plants",
Donetsk, Ukraine, vol.7, p. 59-61.
Gleba D., Kaleda L., Parakony A.,
Borysyuk N., Gleba Y., Negrutiu I. 1987. Nuclear genetics of somatic
cell hybrids. Abstr. 14 Intern. Bot. Congr., West Berlin.
Gleba D., Stupnitsky V. 1988. Use of in vitro culture methods for propagation
of subtropical and green-house plants. Abstr. Int.Conf.
"Biology of cultured cells and biotechnology",
Novosibirsk, Rusia, p. 323-324.
Stupnitsky V., Gleba D. 1988. Introduction of in vitro
morphogenesis in Musa nana. Abstr. Int.Conf. "Biology of
cultured cells and biotechnology", Novosibirsk, Rusia,p.
325-326.
Gleba D., Stupnitsky V. 1989. Propagation of Cuningammia in
in vitro
culture. Abstr. Conf. "Role of Botanical gardens in
preservation and enrichment of world flora", Kiev, vol.2,
p.138.
Stupnitsky V., Gleba D. 1989. Generative multiplication of
Bletilla striota Reinchen. in sterile culture. Abstr. Conf.
"Role of Botanical gardens in preservation and enrichment of
world flora", Kiev, vol.2, p.148.
Gleba D., Stupnitsky V. 1989. Development of technology of
microclonal propagation of higher plants in Hortus Botanicus
Fominianus.
In: "Conservation, study and enrichment of world flora",
Kiev, Ukraine, vol.16, p. 66-70.
Gleba D., Stupnitsky V. 1990. Use of in vitro methods for propagation of
subtropical green-house plants. Abstr. VII Intern. Congr. Plant
Tissue Cell Culture. Amsterdam, A-3-74, p.101.
Gleba D., Stupnitsky V., Gordzievskaya L. 1991.Propagation of
several fern species in vitro. In: "Conservation, study and enrichment of
world flora", Kiev, Ukraine, vol.18, p. 112-117.
Doloressa Gleba, David E. Salt, Robert
Smith, Yoram Kapulnik, Burt Ensley and Ilya Raskin. 1996. Screening
for metal accumulating plants. Abstr. Ann. Meet .of AAPS, San
Antonio, Texas, p. 552.
Ilya Raskin, Doloressa Gleba, Robert Smith, David E. Salt,
Slavik Dushenkov, Yoram Kapulnik and Burt Ensley. 1996. Using plant
seedlings to remove heavy metals from water. Abstr. Ann. Meet .of
AAPS, San Antonio, Texas, p. 552.
David E. Salt, Ingrid J. Pickering, Roger C. Prince, Doloressa
Gleba, Slavik Dushenkov, Robert D. Smith and Ilya Raskin. 1997.
Metal accumulation by aquacultured seedlings of Indian mustard.
Environ. Sci. Tech., 31, p.1636-1644.
Slavik Dushenkov, Dev Vasudev, Yoram Kapulnik, Doloressa
Gleba, David Fleishner, K.C. Ting and Burt Ensley.1997. Removal
of Uranium from water using terrestrial plants. Environ. Sci. Tech.,
31, p.3468-3474.
Gleba D., Gleba, Y., M. Skarzhinskaya,
K. Glimelius and I. Raskin. 1998. Transfer of resistance to heavy
metals from Thlaspi to Brassica by asymmetric hybridization. Abstr.
IX Int. Congr. On Plant Tissue Culture, Jerusalem, p. 145.
Doloressa Gleba, Nicolai V. Borisjuk,
Liudmila G. Borisjuk, Ralf Kneer, Alexander Poulev, Marina
Skarzhinskaya, Slavik Dushenkov, Sithes Logendra, Yuri Y. Gleba and
Ilya Raskin.1999. Use of plant roots for phytoremediation and
molecular farming. Proc. Natl Acad. Sci. USA Vol. 96, pp. 5973-5977.
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