Ljudmila Borysyuk Postdoctoral Associate

Dr. Ljudmila Borysyuk has over 15 years experience of academic and applied research in microbiology, plant molecular physiology and biotechnology. During 1980-1990 Dr. Borysyuk studied physiology of melannin containing fungi under different extreme conditions including the exposure to high radiation after the Chernobyl accident. Since 1991, she shifted her focus to plant molecular biology and physiology, acquiring solid laboratory experience at the University of Tuebingen.  In 1993, Dr. Borysyuk joined the Institute of Plant Genetics, (Gatersleben), where she investigated tissue specific promoter activiites during embryogenesis by in situ hybridization of mRNA and immunodetection of proteins. She designed a pioneer method of high-resolution histographical mapping of glucose concentration in plant tissues. In 1996, Dr. Borysyuk moved to the Biotech Center of Rutgers, working as a research scientist for Phytomedics Inc. She established an original plant cultivation system, designed methods for the deection of protein activities (xylanase, SEAP, GFP) secreted into the hydroponic medium. She significantly contributed to the development of a novel plant transformation system, and thoroughly tested its activity at DNA, mRNA and protein expression levels. PUBLICATIONS: Borisjuk N, Borisjuk L, Logendra S, Petersen F, Gleba Yu, Raskin I: Production of recombinant proteins in plant root exudates. Accepted for publication in Nature Biotechnology (May, 1999). Gleba D, Borisjuk N, Borisjuk L, Kneer R, Poulev A, Skarzhinskaya M, Dushenkov S, Logendra S, Gleba Yu and Raskin I: Radical approaches to phytoremediation and molecular farming. Accepted for publication in PNAS USA, v. 96, (1999). Borisjuk L, Walenta S, Weber H, Mueller-Klieser and Wobus U.High-resolution histographical mapping of glucose concentrations in developing cotyledons of Vicia faba in relation to mitotic activity and storage processes: glucose as a possible developmental trigger. Plant J. (cover story) 15: 583-591, (1998). Weber H, Heim U, Golombek S, Borisjuk L,, Manteuffel R, Wobus U: Expression of a yeast-derived invertase in developing cotyledons of Vicia narbonensis alters the carbohydrate state and affects storage functions. Plant J. 16:163-172 , ( 1998). Weber H, Heim U, Golombek S, Borisjuk L, Wobus U: Assimilate uptake and the regulation of seed development. Seed Science Research In Press (1998) Borisjuk N, L. Sitailo, Borisjuk L, K. Adler, L. Malysheva, A. Tewes and R. Manteuffel: Calreticulin in plant cells: primary structure, developmentally regulated expression and intracellular distribution. Planta 206: 504-514, (1998). Weber H, Golombek S, Heim U, Borisjuk L, Panitz R, Manteuffel R, Wobus U: Integration of carbohydrate and nitrogen metabolism during legume development - implication for storage product synthesis. J. Plant Physiol 152(6):641-648, (1998) Weber H, Borisjuk L, Heim U, Sauer N, Wobus U: A role of sugar transporters during seed development: molecular charecterization of a hexose and a sucrose carrier in faba bean seeds. Plant Cell 9, 895-908 (1997) Weber H, Borisjuk L,, Wobus U: Sugar import and metabolism during seed development (review). Trends in Plant Science 2, 169-174 (1997) Wobus U, Borisjuk L, Heim U, Weber H: Ein Kapitel Metamorphose der Pflanzen; Embryogenese und Samenentwicklung. Leopoldina (R.3) 42: 297-304 (1997) Balzer, H.J. Borisiuk, L. Meyer, H.M. Matzk, F. Baumlein, H. A pollen allergen-encoding gene is expressed in wheat ovaries. Plant Molecular Biology. 32 (3) p. 435-445 (1996). Weber H, Buchner P, Borisjuk L,, Wobus U: Sucrose methabolism during cotyledon development of Vicia faba L. is controlled by the concerted action of both sucrose - phosphate synthase and sucrose synthase: expression pattern, metabolic regulation and implications for seed development. Plant J. 9, 841-850 (1996) Buchner P, Borisjuk L, Wobus U: Glucan phosphorylases in Vicia faba L.: cloning, structural analysis and expression patterns of cytosolic and plastidic forms in relation to starch. Planta, 199, 64-73 (1996). Borisjuk L, Weber H, Heim U, Wobus U: Transfer cell-specific expression of a putative sucrose - H+ symporter in developing embryos of Vicia faba. Plant Physiol. Biochem. (Special issue) 180 (1996) Wobus U, Borisjuk L, Panitz R, Manteuffel R, Bäumlein H, Wohlfarth T, Heim U, Weber H, Misera S, Weschke W: Control of seed storage protein gene expression: New aspects on an old problem. J. Plant Physiol. 145, 592-599 (1995) Weber H, Borisjuk L, Heim U, Wobus U: Seed coat associated invertases of Vicia faba control both unloding and storage functions: cloning of cDNAs and cell-type specific expression. Plant Cell, 7, 1835-1846 (1995) Weber H, Borisjuk L, Wobus U: Cell-type specific, coordinate expression of two ADP-glucose pyrophosphorylase genes in relation to starch biosynthesis during seed development of Vicia faba L. Planta, 105, 352-361 (1995) Panitz R, Borisjuk L, Manteuffel R, Wobus U: Transient expression of storage-protein genes during early embryogenesis of Vicia faba: synthesis and metabolization of vicilin and legumin in the embryo, suspensor and endosperm. Planta 197, 765-774 (1995) Borisjuk L, Weber H, Panitz R, Manteuffel R, Wobus U: Embryogenesis of Vicia faba L.: histo-differentiation in relation to starch and storage protein synthesis. J. Plant Physiol. 147, 203-218 (1995) Borisjuk, N., Borisjuk L, G. Petjuch and V. Hemleben. Comparison of nuclear ribosomal RNA genes among Solanum species and other Solanaceae. Genome 37,271-279 (1994) Schweizer G, Borisjuk N, Borisjuk L, Stadler M, Stelzer T, Schilde-Rentschler L, Hemleben V. Organization and distribution of different repeated genome elements in Solanum and their use as markers for the characterization of species and culrivars. Theor.Appl.Genet.,85: 801-808, (1993) Borisjuk L, Arzatbanov V.Yu., Zdanova N.N. Effect of electron chain inhibitors on the respiration and growth of Cladosporium cladosporioides and its albino mutant. Izvestija Acad. Sci. USSR., 3 (1992). Borisjuk L, Kharatjan E.F., Zdanova N.N. Localization and dinamics of melanine accumulation in cells of Cladosporium cladosporioides (Fresen) de Vries. Microbiol. J.(Russian), 53, 6, 10-16 (1991). Zdanova N.N., Borisjuk L, Arzatbanov V.Yu. Occurence of the K-Type of Life Strategy in some melanin-containing fungi under experimental conditions. Folia Microbiol., 35, 423-430 (1990). Zdanova N.N., Rozshko I.I., Borisjuk L. Assimilation of radioactive carbon from 14CO2 by Cladosporium cladosporioides in dark and on the light. Mycol. Phytopatol., 19, 2, 132-139 (1985). ABSTRACTS: Borisjuk N, Borisjuk L, . Hemleben V, Gleba Y. and Raskin I. 1998. Heterologous gene amplification using rDNA intergenic spacer sequence. Abstracts of the 2-nd International Symposium on Plant Biotechnology, Kiev, 4-8 Oktober, Ukraine, p.26. Borisjuk L, Borisjuk N, Gleba Yu, Raskin I. 1998. Secretion of transgenic proteins by plant roots. Abstracts of the IX International congress on plant tissue and Cell Culture, Jerusalem, 14-19 June, Israel, p.39. Borisjuk L, Walenta S, Weber H, Heim U, Panitz R, Manteuffel R, Mueller-Klieser W and Wobus U. 1997.Developmental patterns of storage product accumulation and their relationship to temporal and spatial changes in the sugar status of faba bean seeds . 7th International Seed protein Symposium , Gatersleben, Germany, 23-27 june , p.27. Weber H,Heim U, Borisjuk L, Golombek S and Wobus U.1997.Metabolik control of legume seed development: the role of invertases, sugar transporters and sugar gradients. 5th International Congress of Planr Molecular Biology, 21-27 september,Singapore, p.401. Borisjuk N, Adler K, Borisjuk L, Sitailo L, Tewes A, Manteuffel R. 1997. Is calreticulin involved into the secretion pathway? Abstracts of the International Conference "The Plant Secretory System: Mechanisms, Pathways and Applications in Biotechnology", York, 2-5 July, England, P10. Borisjuk L, Weber H, Heim U and Wobus U.1996.Transfer cell-specific expression of a putative sucrose - H+ symporter in developing embryos of Vicia faba. The 10th FESPP Congress, 9-13 September , Firence, Italy. Weber H, Borisjuk L, Wobus U. 1996 . Apoplastische Invertase der Samenschale ist an der Kontrolle der Zellteilungsphase waehrend der Embryonalentwicklung von Vicia faba beteilight . 9th Tagung Molekularbiologie der Pflancen, 5-8 Marz, Wernigerode, Germany, Borisjuk N, L. Borisjuk, V. Hemleben, Y. Gleba and I. Raskin. 1998. Heterologous gene amplification using rDNA intergenic spacer sequence. Abstracts of the 2-nd International Symposium on Plant Biotechnology, Kiev, 4-8 Oktober, Ukraine. Borisjuk N, Borisjuk L, Hemleben V. 1993. rDNA comparison among Solanum species and other Solanaceae. Abstracts of the XVII-th International Congress of Genetics, Birmingham, 15-21 August, p. 192 Borisjuk N, Borisjuk L, Hemleben V. 1992. Ribosomal RNA genes in the genus Solanum. In: New Trends in Genetics. Annual Meeting of the Genetic Society, 27-30 September, Tuebingen, p. 119 Borisjuk N, Borisjuk L, Hemleben V. 1991. Evolutionary relationships and organization of nuclear ribosomal RNA genes in wild species and cultivars of the genus Solanum. Abstracts of the Third International Confress of Plant Molecular Biology. Tucson, 6-11 October, USA Borisjuk N., Borisjuk L, Hemleben V. 1991. Organization of nuclear ribosomal RNA genes in species of the genus Solanum and nucleotide sequence of the S. tuberosum intergenic spacer. Abstracts of the 2-nd International Potato Molecular Biology Symposium. St. Andrews, 11-15 August, Scotland Borisjuk N, Borisjuk L, Hemleben V. 1991. Evolutionary relationships between wild species and cultivars of potato shown by repetitive sequences. Abstracts of the Eucarpia Symposium on Genetic Manipulation in Plant Breeding, Reus/Salou, May 25-30, IV.p. 15 Borisjuk L. 1989. Physiology of the melanin-producing fungi strain. 1989. 4th research conference in Biology and biotechnology , 5-12 june, Tashkent, p.18. U.S. PATENT M.Borysyuk, L. Borysyuk, I. Raskin. Materials and methods for amplifying polynucleotides in plants. Docked No: 29155/34699, filed on June 15, 1998. INVITED LECTURES: Transfer cell-specific expression of a putative sucrose - H+ symporter in dveloping embryos of Vicia faba. The 10th FESPP Congress, Firenze, Italy, September 9-13, 1996. Secretion of transgenic proteins by plant roots. IX International congress on plant tissue and Cell Culture, Jerusalem, Israel 14 - 19 June, 1998.