Ph.D., The Rockefeller University, 1998
Professional Summary/CV [.PDF]
Department of Biological Sciences, Arts and Sciences, Newark; Rutgers
Areas of Interest
Advanced Cell Biology
Memberships and Professional Service
Reviewer for articles in Journal of Cell Science, Molecular Biology of the Cell; Lecturer for Micron, UK, 2008; FASEB Conference, 2007; Gordon Conference Viruses and Cells, 2007; American Society of Human Genetics, 2005; Zeiss Confocal/2 Photon Advanced Imaging Scientific Symposium; Gorden Conference Molecular Membrane Biology, 2003; American Society of Cell Biology, 2002 & 2001.
Academic Interests and Plans
My expertise lies in the development and application of a variety of live-cell imaging tools to important cell biological problems such as multidrug resistance development in tumor cells, organelle replication during mitosis, and the dynamics of viral replication. Over the past 10 years I have gained expertise in the use of single and multiphoton confocal microscopy, fluorescence correlation spectroscopy and other light-based methods (FRAP, FRET, Ratio imaging) and published highly cited papers using these tools. I have trained in the laboratories of Dr. Sanford Simon at Rockefeller University (1992-1998) and Dr. Jennifer Lippincott-Schwartz at the NIH (1999-2005), two labs that are at the forefront in the development and application of live-cell imaging methodologies.
Since 2006 I have my own independent tenure-track position as Assistant Professor in the Department of Biology at Rutgers University. Here I have developed a research group that is focused on studying viral replication in living cells using novel imaging methodologies. Experimental approaches to the study of viral replication have been studied primarily by methods that involve either in vitro assays involving cell extracts and viral nucleic acids; histochemistry; or macroscopic ensemble measurements that average information on the replication kinetics and the role of particular cellular machinery from many millions of virally infected cells i.e., PCR, cell fractionation, RNA/protein blotting based methods. However live-cell imaging methodologies are particularly well suited to the study of the virus-cell interface because they can provide valuable spatio-temporal information on the sequence of events without perturbing the events themselves and furthermore allow investigation of virus host events at the single-cell level. Our studies will provide valuable information on the generation, organization and kinetics of replication sites at the single-cell level and the application of molecular beacons to study viral replication in living cells can be generalized to many other virus-cell systems.