Plasposon Information Page


What are plasposons?
Plasposons are mini-transposons containing an origin of replication between the inverted repeats. Thus the plasmid portion of the molecule moves with the transposon to a new location. These artificially derived plasmid transposons are dubbed plasposons.

How useful are plasposons?
Once a mutant has been constructed with a plasposon, the region around the insertion point can be rapidly cloned and sequenced. The insertion point can be mapped by PFGE using the rare restriction enzyme cutting sites built into the molecule. Several plasposon insertions can be pooled to rapidly construct a genomic library of the target strain.

How do I do this?
The procedures can easily be performed as shown in this diagram.

How can I access the nucleotide sequences of the plasposons and the antibiotic resistant cassettes?
The individual sequences that can be accessed are the:


Do you have any fancy color maps that I can download?
Currently, we have two color maps which can be downloaded: pTnMod-OGm and p34S.

Can I get a reprint of the Applied and Environmental Microbiology Paper?
Reprints are available for download from the ASM web site.

How can I get these plasposons?
Download and print the PDF or HTML request form and mail it to us.


Any other questions?
Please contact either Jonathan J. Dennis or Gerben J. Zylstra for more information.